Sphingosine-1-phosphate as a ligand for the G protein-coupled receptor EDG-1 Academic Article Article uri icon

Overview

MeSH Major

  • Lysophospholipids
  • Receptors, Lysophosphatidic Acid
  • Sphingosine

abstract

  • The sphingolipid metabolite sphingosine-1-phosphate (SPP) has been implicated as a second messenger in cell proliferation and survival. However, many of its biological effects are due to binding to unidentified receptors on the cell surface. SPP activated the heterotrimeric guanine nucleotide binding protein (G protein)-coupled orphan receptor EDG-1, originally cloned as Endothelial Differentiation Gene-1. EDG-1 bound SPP with high affinity (dissociation constant = 8.1 nM) and high specificity. Overexpression of EDG-1 induced exaggerated cell-cell aggregation, enhanced expression of cadherins, and formation of well-developed adherens junctions in a manner dependent on SPP and the small guanine nucleotide binding protein Rho.

publication date

  • March 6, 1998

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1126/science.279.5356.1552

PubMed ID

  • 9488656

Additional Document Info

start page

  • 1552

end page

  • 5

volume

  • 279

number

  • 5356