Phosphatidylinositol-3,4,5-trisphosphate (Ptdlns-3,4,5-P3)/Tec kinase-dependent calcium signaling pathway: A target for SHIP-mediated inhibitory signals Academic Article uri icon


MeSH Major

  • Calcium
  • Phosphatidylinositol Phosphates
  • Phosphoproteins
  • Phosphoric Monoester Hydrolases
  • Protein-Tyrosine Kinases
  • Signal Transduction


  • Tec family non-receptor tyrosine kinases have been implicated in signal transduction events initiated by cell surface receptors from a broad range of cell types, including an essential role in B-cell development. A unique feature of several Tec members among known tyrosine kinases is the presence of an N-terminal pleckstrin homology (PH) domain. We directly demonstrate that phosphatidylinositol-3,4,5-trisphosphate (PtdIns-3,4,5-P3) interacting with the PH domain acts as an upstream activation signal for Tec kinases, resulting in Tec kinase-dependent phospholipase Cgamma (PLCgamma) tyrosine phosphorylation and inositol trisphosphate production. In addition, we show that this pathway is blocked when an SH2-containing inositol phosphatase (SHIP)-dependent inhibitory receptor is engaged. Together, our results suggest a general mechanism whereby PtdIns-3,4,5-P3 regulates receptor-dependent calcium signals through the function of Tec kinases.

publication date

  • April 1998



  • Academic Article



  • eng

PubMed Central ID

  • PMC1170542

Digital Object Identifier (DOI)

  • 10.1093/emboj/17.7.1961

PubMed ID

  • 9524119

Additional Document Info

start page

  • 1961

end page

  • 72


  • 17


  • 7