Role of oxidants in influenza virus-induced gene expression. Academic Article uri icon

Overview

MeSH

  • Acetylcysteine
  • Animals
  • Base Sequence
  • Binding Sites
  • Chick Embryo
  • Epithelial Cells
  • Humans
  • Interleukin-8
  • Lung Neoplasms
  • NF-kappa B
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Sp1 Transcription Factor
  • Transcription, Genetic
  • Tumor Cells, Cultured

MeSH Major

  • Gene Expression Regulation
  • Influenza A virus
  • Nuclear Proteins
  • Reactive Oxygen Species
  • Superoxide Dismutase

abstract

  • Influenza virus-induced epithelial damage may be mediated, in part, by reactive oxygen intermediates (ROIs). In this study, we investigated the role of ROIs in the influenza virus-induced gene expression of antioxidant enzymes and in the activation of nuclear factor-kappa B (NF-kappa B), an oxidant-sensitive transcriptional factor. Influenza virus infection increased production of intracellular ROIs in A549 pulmonary epithelial cells. Induction of manganese superoxide dismutase (MnSOD) mRNA correlated with increased MnSOD protein and enzyme activity. Influenza virus infection also activated NF-kappa B binding as determined by an electrophoretic mobility shift assay. Pretreatment of A549 cells with N-acetyl-L-cysteine attenuated virus-induced NF-kappa B activation and interleukin (IL)-8 mRNA induction but did not block induction of MnSOD mRNA. In contrast, pyrrolidine dithiocarbamate blocked activation of NF-kappa B and induction of MnSOD and IL-8 mRNAs. Treatment with pyrrolidine dithiocarbamate also markedly decreased virus-induced cell death. Thus oxidants are involved in influenza virus-induced activation of NF-kappa B, in the expression of IL-8 and MnSOD, and in virus-induced cell death.

publication date

  • January 1998

has subject area

  • Acetylcysteine
  • Animals
  • Base Sequence
  • Binding Sites
  • Chick Embryo
  • Epithelial Cells
  • Gene Expression Regulation
  • Humans
  • Influenza A virus
  • Interleukin-8
  • Lung Neoplasms
  • NF-kappa B
  • Nuclear Proteins
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Reactive Oxygen Species
  • Sp1 Transcription Factor
  • Superoxide Dismutase
  • Transcription, Genetic
  • Tumor Cells, Cultured

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 9458811

Additional Document Info

start page

  • L134

end page

  • L142

volume

  • 274

number

  • 1 Pt 1