Construction and characterization of hexon-chimeric adenoviruses: specification of adenovirus serotype. Academic Article uri icon

Overview

MeSH

  • Adenoviridae Infections
  • Animals
  • Antibodies, Viral
  • Antigens, Viral
  • Cell Line
  • Cross Reactions
  • Epitopes
  • Genes, Viral
  • Genetic Vectors
  • Humans
  • Neutralization Tests
  • Rats
  • Rats, Sprague-Dawley
  • Recombination, Genetic
  • Serotyping
  • Transduction, Genetic

MeSH Major

  • Capsid
  • Capsid Proteins
  • Chimera
  • Mastadenovirus

abstract

  • This study has used the strategy of gene replacement to characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon gene with sequences from Ad2, we have changed the type specificity of the chimeric virus. The type-determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop 2. In spite of the serotype distinctiveness of the chimeric hexon viruses, epitope similarity between the vectors resulted in a low level of cross-reactive neutralizing antibody, which in combination with activated cellular and innate arms of the immune system is sufficient to suppress gene transduction following readministration in vivo.

publication date

  • December 1998

has subject area

  • Adenoviridae Infections
  • Animals
  • Antibodies, Viral
  • Antigens, Viral
  • Capsid
  • Capsid Proteins
  • Cell Line
  • Chimera
  • Cross Reactions
  • Epitopes
  • Genes, Viral
  • Genetic Vectors
  • Humans
  • Mastadenovirus
  • Neutralization Tests
  • Rats
  • Rats, Sprague-Dawley
  • Recombination, Genetic
  • Serotyping
  • Transduction, Genetic

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC110610

PubMed ID

  • 9811772

Additional Document Info

start page

  • 10260

end page

  • 10264

volume

  • 72

number

  • 12