Augmentation of blood platelet levels by intratracheal administration of an adenovirus vector encoding human thrombopoietin cDNA. Academic Article uri icon

Overview

MeSH

  • Adenoviridae
  • Animals
  • Bone Marrow Cells
  • Cell Line
  • Cytomegalovirus
  • DNA, Complementary
  • Genetic Therapy
  • Genetic Vectors
  • Hematopoietic Stem Cells
  • Humans
  • Megakaryocytes
  • Mice
  • Mice, Inbred BALB C
  • Mutagenesis, Insertional
  • Recombinant Proteins
  • Spleen

MeSH Major

  • Gene Transfer Techniques
  • Platelet Count
  • Thrombopoietin

abstract

  • This study was designed to evaluate the hypothesis that administration of a replication-deficient, recombinant adenovirus vector to the epithelial surface of the respiratory tract can be used to deliver a recombinant protein to the systemic circulation in sufficient quantities to evoke a systemic response appropriate to the recombinant protein. We administered AdCMV.TPO-an adenovirus vector containing an expression cassette coding for the human thrombopoietin (TPO) cDNA-to the respiratory epithelium of immunocompetent Balb/c mice. Over the following week, serum human TPO levels were elevated, platelet levels increased more than sixfold, and megakaryocytosis was evident in bone marrow. This strategy may be a useful approach to the nonparenteral administration of a variety of therapeutic recombinant proteins, such as those relevant to clotting, endocrine function, and bone-marrow function.

publication date

  • June 1997

has subject area

  • Adenoviridae
  • Animals
  • Bone Marrow Cells
  • Cell Line
  • Cytomegalovirus
  • DNA, Complementary
  • Gene Transfer Techniques
  • Genetic Therapy
  • Genetic Vectors
  • Hematopoietic Stem Cells
  • Humans
  • Megakaryocytes
  • Mice
  • Mice, Inbred BALB C
  • Mutagenesis, Insertional
  • Platelet Count
  • Recombinant Proteins
  • Spleen
  • Thrombopoietin

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1038/nbt0697-570

PubMed ID

  • 9181581

Additional Document Info

start page

  • 570

end page

  • 573

volume

  • 15

number

  • 6