Augmentation of blood platelet levels by intratracheal administration of an adenovirus vector encoding human thrombopoietin cDNA Academic Article uri icon

Overview

MeSH Major

  • Gene Transfer Techniques
  • Platelet Count
  • Thrombopoietin

abstract

  • This study was designed to evaluate the hypothesis that administration of a replication-deficient, recombinant adenovirus vector to the epithelial surface of the respiratory tract can be used to deliver a recombinant protein to the systemic circulation in sufficient quantities to evoke a systemic response appropriate to the recombinant protein. We administered AdCMV.TPO-an adenovirus vector containing an expression cassette coding for the human thrombopoietin (TPO) cDNA-to the respiratory epithelium of immunocompetent Balb/c mice. Over the following week, serum human TPO levels were elevated, platelet levels increased more than sixfold, and megakaryocytosis was evident in bone marrow. This strategy may be a useful approach to the nonparenteral administration of a variety of therapeutic recombinant proteins, such as those relevant to clotting, endocrine function, and bone-marrow function.

publication date

  • June 1997

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 9181581

Additional Document Info

start page

  • 570

end page

  • 3

volume

  • 15

number

  • 6