Clinical application of molecular biology: A study of allograft rejection with polymerase chain reaction Academic Article uri icon

Overview

MeSH Major

  • Gene Expression
  • Graft Rejection
  • Kidney Transplantation

abstract

  • This article explores the clinical usefulness of reverse transcriptase-polymerase chain reaction in organ graft recipients. In this study, reverse transcriptase-polymerase chain reaction was used to identify intrarenal expression of cytotoxic attack molecules (granzyme B and perforin) and immunoregulatory cytokines (IL-2, IL-4, IL-10, IFN-gamma, and TGF-beta 1) in human renal allograft biopsies. The biopsies (n = 127) were classified using the Banff criteria, and intrarenal gene expression was correlated with the histologic diagnosis. Molecular analyses revealed that intragraft display of mRNA encoding granzyme B, IL-10, or IL-2 is a correlate of acute rejection, and intrarenal expression of TGF beta 1 mRNA is a correlate of chronic rejection. In addition to demonstrating differential and highly selective intragraft gene expression during rejection, these data suggest that therapeutic strategies directed at the molecular correlates of rejection might refine existing antirejection strategies.

publication date

  • May 21, 1997

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 9145034

Additional Document Info

start page

  • 264

end page

  • 7

volume

  • 313

number

  • 5