Construction of an adenovirus type 7a E1A- vector. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cells, Cultured
  • Defective Viruses
  • Gene Expression Regulation, Viral
  • Gene Transfer Techniques
  • Humans
  • Mice
  • Transduction, Genetic
  • Virus Replication

MeSH Major

  • Adenovirus E1A Proteins
  • Adenoviruses, Human
  • Genetic Vectors

abstract

  • A strategy for constructing replication-defective adenovirus vectors from non-subgroup C viruses has been successfully demonstrated with adenovirus type 7 strain a (Ad7a) as the prototype. An E1A-deleted Ad7a reporter virus expressing the chloramphenicol acetyltransferase (CAT) gene from the cytomegalovirus promoter enhancer was constructed with DNA fragments isolated from Ad7a, an Ad7a recombination reporter plasmid, and the 293 cell line. The Ad7a-CAT virus particle transduces A549 cells as efficiently as Ad5-based vectors. Intravenous infections in a murine model indicate that the Ad7a-CAT virus infects a variety of tissues, with maximal levels of CAT gene expression found in the liver. The duration of Ad7a-CAT transgene expression in the liver was maximally maintained 2 weeks postinfection, with a decline to baseline activity by the week 4 postinfection. Ad7a-CAT represents the first example of a non-subgroup C E1A- adenovirus gene transfer vector.

publication date

  • November 1997

has subject area

  • Adenovirus E1A Proteins
  • Adenoviruses, Human
  • Animals
  • Cells, Cultured
  • Defective Viruses
  • Gene Expression Regulation, Viral
  • Gene Transfer Techniques
  • Genetic Vectors
  • Humans
  • Mice
  • Transduction, Genetic
  • Virus Replication

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC192370

PubMed ID

  • 9343264

Additional Document Info

start page

  • 8946

end page

  • 8951

volume

  • 71

number

  • 11