Adenovirus-mediated gene transfer of fibroblast growth factor-1: angiogenesis and tumorigenicity in nude mice. Academic Article uri icon

Overview

MeSH

  • Animals
  • Gene Expression Regulation, Neoplastic
  • Genetic Vectors
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mice, Nude

MeSH Major

  • 3T3 Cells
  • Adenoviridae
  • Fibroblast Growth Factor 1
  • Gene Transfer Techniques
  • Neoplasms, Experimental
  • Neovascularization, Pathologic

abstract

  • Gene transfer of angiogenic growth factors with replication-deficient recombinant adenovirus (Ad) vectors may provide a new approach to the treatment of ischemic diseases. To determine if Ad-infected cells could stimulate angiogenesis in vivo and to assess the tumorigenicity of cells infected with these vectors, NIH3T3 fibroblasts infected with Ad vectors coding for human acidic fibroblast growth factor (aFGF-1) were used in angiogenic and tumorigenic assays. Infected cells induced a strong angiogenic response in vivo, while cells infected with control virus did not. Stable 3T3 transfectants expressing the FGF-1 gene were also highly angiogenic and exhibited growth in soft agar, while Ad-infected cells did not. Ad-infected cells grew transiently in nude mice, whereas 3T3 transfectants formed large tumors which grew exponentially. Extrapolation of cell dose-response curves showed that a minimum of 1.5 x 10(4) infected cells were required for transient tumor cell growth in vivo. Ad-infected cells cultured in vitro for 30 days lost their invasive phenotype and the ability for transient cell growth in nude mice. Thus, phenotypic changes induced by Ad-mediated gene transfer of FGF-1 are transient both in vitro and in vivo, suggesting that these Ad vectors do not have tumorigenic potential. Stimulation of angiogenesis by Ad-infected cells may be useful for the evaluation of anti-angiogenic and anti-tumor agents.

publication date

  • October 9, 1997

has subject area

  • 3T3 Cells
  • Adenoviridae
  • Animals
  • Fibroblast Growth Factor 1
  • Gene Expression Regulation, Neoplastic
  • Gene Transfer Techniques
  • Genetic Vectors
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mice, Nude
  • Neoplasms, Experimental
  • Neovascularization, Pathologic

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 9335452

Additional Document Info

start page

  • 258

end page

  • 263

volume

  • 73

number

  • 2