Antiserum against Escherichia coli J5 contains antibodies reactive with outer membrane proteins of heterologous gram-negative bacteria Academic Article uri icon

Overview

MeSH Major

  • Antibodies, Bacterial
  • Bacterial Outer Membrane Proteins
  • Escherichia coli
  • Immune Sera

abstract

  • The binding of IgG in antiserum to Escherichia coli J5 to the surface of Enterobacteriaceae and to cell wall fragments released from serum-exposed bacteria was studied in a search for potentially protective epitopes other than lipopolysaccharide (LPS). IgG titers to multiple heterologous gram-negative smooth bacteria increased following incubation of the bacteria in serum and decreased following absorption with serum-exposed heterologous bacteria. IgG eluted from absorbing bacteria bound to at least three conserved bacterial outer membrane proteins (OMPs), but not LPS, as assessed by immunoblotting. The same OMPs were present in LPS-containing macromolecular cell wall fragments released by incubation of heterologous gram-negative bacteria in human serum. Part of the protection offered by J5 antiserum could be from binding of IgG to conserved OMPs at the bacterial surface or to OMPs in cell-wall fragments released from dying bacteria.

publication date

  • November 6, 1997

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 9359727

Additional Document Info

start page

  • 1260

end page

  • 8

volume

  • 176

number

  • 5