Uptake of fluorescent dyes associated with the functional expression of the cystic fibrosis transmembrane conductance regulator in epithelial cells Academic Article Article uri icon


MeSH Major

  • Gene Expression
  • Lung Diseases
  • Mutation
  • Respiration
  • Smoking


  • Specific mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), the most common autosomal recessive fatal genetic disease of Caucasians, result in the loss of epithelial cell adenosine 3',5'-cyclic-monophosphate (cAMP)-stimulated Cl- conductance. We show that the influx of a fluorescent dye, dihydrorhodamine 6G (dR6G), is increased in cells expressing human CFTR after retrovirus- and adenovirus-mediated gene transfer. dR6G influx is stimulated by cAMP and is inhibited by antagonists of cAMP action. Dye uptake is ATP-dependent and inhibited by Cl- removal or the addition of 10 mM SCN-. Increased staining is associated with functional activation of CFTR Cl- permeability. dR6G staining enables both the fluorescent assessment of CFTR function and the identification of successfully corrected cells after gene therapy.

publication date

  • February 6, 1996



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1073/pnas.93.3.1167

PubMed ID

  • 8577734

Additional Document Info

start page

  • 1167

end page

  • 72


  • 93


  • 3