Limited heterogeneity of biased T-cell receptor V beta gene usage in lung but not blood T cells in active pulmonary sarcoidosis. Academic Article uri icon

Overview

MeSH

  • Adult
  • Amino Acid Sequence
  • Base Sequence
  • Bronchoalveolar Lavage Fluid
  • Female
  • Humans
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction

MeSH Major

  • CD4-Positive T-Lymphocytes
  • Lung
  • Receptors, Antigen, T-Cell, alpha-beta
  • Sarcoidosis, Pulmonary

abstract

  • Sarcoidosis is a multisystem disorder characterized by non-caseating granulomas and the accumulation of CD4+ T cells in involved tissues such as the lung. To evaluate the diversity of the CD4+ T-cell repertoire in this disorder, a detailed clonal analysis was performed in five individuals with active sarcoidosis who demonstrated preferential accumulation of T cells expressing the T-cell receptor variable gene family V beta 8 in either the lung or blood. In three individuals, analysis of unselected samples of nucleotide sequences derived from V beta 8+ lung T cells demonstrated degrees of clonality ranging from 11% to 46%, indicating the expansion of limited numbers of V beta 8+ T-cell clones in the lung. Analysis of the corresponding deduced amino acid sequences demonstrated common VDJ junctional amino acid residues in the dominant V beta 8+ T-cell clones derived from two oligoclonal V beta 8+ lung T-cell populations, consistent with an antigen-specific T-cell response. In contrast, analysis of V beta 8+ CD4+ T cells from the blood of an individual with a marked bias for peripheral blood V beta 8+ T cells demonstrated no evidence of oligoclonality, suggesting that the stimulus for circulating biased V beta-specific T cells in sarcoidosis may derive from a different, perhaps superantigenic, origin. Clinical improvement in the disease either in response to treatment with corticosteroids or as a result of spontaneous resolution was associated with a decrease in the proportion of V beta 8-specific T cells in the biased lung and/or blood T-cell compartments. Together, these observations are consistent with a role for this T-cell subset in the clinical manifestations of active granulomatous disease.

publication date

  • August 1996

has subject area

  • Adult
  • Amino Acid Sequence
  • Base Sequence
  • Bronchoalveolar Lavage Fluid
  • CD4-Positive T-Lymphocytes
  • Female
  • Humans
  • Lung
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell, alpha-beta
  • Sarcoidosis, Pulmonary

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC1456627

PubMed ID

  • 8881751

Additional Document Info

start page

  • 516

end page

  • 523

volume

  • 88

number

  • 4