Ultrastructural evidence for altered calcium in motor nerve terminals in amyotrophic lateral sclerosis Academic Article uri icon


MeSH Major

  • Amyotrophic Lateral Sclerosis
  • Calcium
  • Motor Neurons
  • Nerve Endings


  • Numerous studies of amyotrophic lateral sclerosis have suggested that increased intracellular calcium is a common denominator in motoneuron injury. In experimental models, IgG from patients with amyotrophic lateral sclerosis enhanced calcium entry and induced apoptotic cell death in vitro as well as increased intracellular calcium and induced ultrastructural alterations of the motor nerve terminals in mice in vivo. To determine whether similar increases in intracellular calcium and altered morphology are present in motor nerve terminals of amyotrophic lateral sclerosis patients in vivo, muscle biopsy specimens from 7 patients with amyotrophic lateral sclerosis, 10 nondenervating disease control subjects, and 5 patients with denervating neuropathies were analyzed with ultrastructural techniques, employing oxalate-pyroantimonate fixation to preserve in situ calcium distribution. Motor nerve terminals from amyotrophic lateral sclerosis specimens contained significantly increased calcium, increased mitochondrial volume, and increased numbers of synaptic vesicles compared to any of the disease control groups, without exhibiting excess Schwann envelopment specific to denervating terminals. These results parallel the effect of amyotrophic lateral sclerosis IgG passively transferred to mice, and provide the first demonstration that neuronal calcium is, in fact, increased in amyotrophic lateral sclerosis in vivo.

publication date

  • March 13, 1996



  • Academic Article



  • eng

Digital Object Identifier (DOI)

  • 10.1002/ana.410390210

PubMed ID

  • 8967752

Additional Document Info

start page

  • 203

end page

  • 16


  • 39


  • 2