In vivo replication-deficient adenovirus vector-mediated transduction of the cytosine deaminase gene sensitizes glioma cells to 5-fluorocytosine. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cell Division
  • Cell Line, Transformed
  • Cytosine Deaminase
  • Humans
  • Male
  • Rats
  • Rats, Inbred F344
  • Virus Replication

MeSH Major

  • Adenoviruses, Human
  • Defective Viruses
  • Flucytosine
  • Genetic Vectors
  • Gliosarcoma
  • Nucleoside Deaminases
  • Transduction, Genetic

abstract

  • Viral vector-mediated transfer of chemosensitization genes represents a promising new approach to the treatment of cancer. Previous reports have demonstrated that transfection of the bacterial cytosine deaminase (cd) gene into mammalian cells can sensitize them to the otherwise nontoxic nucleoside, 5-fluorocytosine (5-FC). We now report that a replication-deficient adenovirus vector that transduces the cd gene (Ad.CMV-cd) highly sensitizes 9L gliosarcoma cells to 5-FC, and that gene transduction is associated with a potent bystander effect that is not dependent on direct cell-to-cell contact. Stereotactic injection of Ad.CMV-cd into established rat gliomas, followed by systemic administration of 5-FC in vivo, results in prolongation of survival.

publication date

  • April 10, 1996

has subject area

  • Adenoviruses, Human
  • Animals
  • Cell Division
  • Cell Line, Transformed
  • Cytosine Deaminase
  • Defective Viruses
  • Flucytosine
  • Genetic Vectors
  • Gliosarcoma
  • Humans
  • Male
  • Nucleoside Deaminases
  • Rats
  • Rats, Inbred F344
  • Transduction, Genetic
  • Virus Replication

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1089/hum.1996.7.6-713

PubMed ID

  • 8919593

Additional Document Info

start page

  • 713

end page

  • 720

volume

  • 7

number

  • 6