Expression of calbindin-D28K in motoneuron hybrid cells after retroviral infection with calbindin-D28K cDNA prevents amyotrophic lateral sclerosis IgG-mediated cytotoxicity Academic Article uri icon

Overview

MeSH Major

  • Amyotrophic Lateral Sclerosis
  • Cytotoxicity, Immunologic
  • Immunoglobulin G
  • Nerve Tissue Proteins
  • S100 Calcium Binding Protein G

abstract

  • Calbindin-D28K and/or parvalbumin appear to influence the selective vulnerability of motoneurons in amyotrophic lateral sclerosis (ALS). Their immunoreactivity is undetectable in motoneurons readily damaged in human ALS, and in differentiated motoneuron hybrid cells [ventral spinal cord (VSC 4.1 cells)] that undergo calcium-dependent apoptotic cell death in the presence of ALS immunoglobulins. To provide additional evidence for the role of calcium-binding proteins in motoneuron vulnerability, VSC 4.1 cells were infected with a retrovirus carrying calbindin-D28K cDNA under the control of the promoter of the phosphoglycerate kinase gene. Differentiated calbindin-D28K cDNA-infected cells expressed high calbindin-D28K and demonstrated increased resistance to ALS IgG-mediated toxicity. Treatment with calbindin-D28K antisense oligodeoxynucleotides, which significantly decreased calbindin-D28K expression, rendered these cells vulnerable again to ALS IgG toxicity.

publication date

  • June 25, 1996

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC39107

PubMed ID

  • 8692898

Additional Document Info

start page

  • 6796

end page

  • 801

volume

  • 93

number

  • 13