Retrograde transfer of replication deficient recombinant adenovirus vector in the central nervous system for tracing studies. Academic Article uri icon

Overview

MeSH

  • Afferent Pathways
  • Animals
  • Axonal Transport
  • DNA, Recombinant
  • DNA, Viral
  • Defective Viruses
  • Gene Transfer Techniques
  • Lac Operon
  • Male
  • Microinjections
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Fusion Proteins
  • Replicon
  • beta-Galactosidase

MeSH Major

  • Adenoviruses, Human
  • Brain
  • Corpus Callosum
  • Genetic Vectors
  • Neostriatum

abstract

  • We assessed the application of a replication deficient recombinant adenovirus vector as a retrograde tracer in neural pathway studies. The adenovirus vector, Ad. RSV betagal, containing the intracellular marker gene, beta-galactosidase, was injected directly into the laterodorsal striatum of rats. The retrograde transport of the vector from the injection site was clearly visible in the cerebral cortex, thalamic nucleus, and substantia nigra. No evidence for anterograde transport of the vector was found. When the vector was injected into the genu of the corpus callosum, little uptake of the vector by fibers was noted which suggested that uptake by fibers-of-passage should not be a problem in tracing studies. The present study demonstrates that adenoviral vectors can be useful retrograde tracers in the study of afferent connections within the central nervous system.

publication date

  • December 24, 1995

has subject area

  • Adenoviruses, Human
  • Afferent Pathways
  • Animals
  • Axonal Transport
  • Brain
  • Corpus Callosum
  • DNA, Recombinant
  • DNA, Viral
  • Defective Viruses
  • Gene Transfer Techniques
  • Genetic Vectors
  • Lac Operon
  • Male
  • Microinjections
  • Neostriatum
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Fusion Proteins
  • Replicon
  • beta-Galactosidase

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 8821730

Additional Document Info

start page

  • 31

end page

  • 38

volume

  • 705

number

  • 1-2