Haplotypes of the human β-like globin gene cluster: Analyzing the 3' Hpa I polymorphic site by long distance PCR Academic Article Article uri icon

Overview

MeSH Major

  • Chelation Therapy
  • Iron Chelating Agents
  • Iron Overload

abstract

  • Haplotypes of the beta-like globin gene cluster in individuals with sickle cell disease appear to be important as prognostic factors for the severity of the disease. The Hpa I polymorphic site 3' of the beta-globin gene is very often involved in haplotyping. However, this restriction site cannot be ascertained by conventional PCR, as it is in the middle of a long repetitive sequence. Long distance PCR was employed to amplify this Hpa I-containing region. By positioning either one or both primers outside the repetitive sequence, specific amplification was achieved. Moreover, sufficient amplification can be obtained by using a white cell lysate instead of purified DNA, thereby significantly reducing the amount of blood needed, and the total procedure time.

publication date

  • September 18, 1995

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1002/ajh.2830500112

PubMed ID

  • 7668226

Additional Document Info

start page

  • 57

end page

  • 9

volume

  • 50

number

  • 1