Measurement of vascular permeability in spinal cord using Evans Blue spectrophotometry and correction for turbidity Academic Article Article uri icon

Overview

MeSH Major

  • Brain
  • Nerve Regeneration
  • Neurons
  • Stem Cell Transplantation
  • Stem Cells

abstract

  • Vascular permeability can be visualized by Evans Blue (EB) extravasation and quantified by spectrophotometry after formamide extraction of the tissue. However, formamide extracts show significant turbidity, which may contribute to the total optical density at the wavelength of measurement (e.g., 620 lambda). We developed a simple method for estimating the component of the total optical density of a dyed specimen contributed by turbidity. Our method, which uses a determination of turbidity made at another point of the light spectrum (740 lambda), was more precise than two other EB quantification techniques. We therefore recommend it for individual correction of formamide extracts of spinal cord specimens. The application of this technique to the brain remains to be determined.

publication date

  • January 1995

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0165-0270(94)00172-D

PubMed ID

  • 7475223

Additional Document Info

start page

  • 167

end page

  • 71

volume

  • 58

number

  • 1-2