β1-6 branching of N-linked carbohydrate is associated with K-ras mutation in human colon carcinoma cell lines Academic Article uri icon

Overview

MeSH Major

  • Carbohydrates
  • Colonic Neoplasms
  • Genes, ras
  • Mutation

abstract

  • Here the K-ras genotypes of nine colon carcinoma cell lines are compared to the protein glycosylation patterns found in these cells. By a variety of methodologies utilizing lectins to probe carbohydrate structure, we find evidence that five out of six cell lines having K-ras mutations have elevated amounts of beta 1-6 branching at the trimannosyl core of N-linked carbohydrate. None of the three K-ras wild type cell lines assayed have evidence of elevated beta 1-6 branching. In five out of five cell lines examined, the amount of beta 1-6 branching correlates with the extent of cellular ras-GTP elevation and supports the hypothesis that expression of beta 1-6 branching in colon carcinoma cell lines is quantitatively linked to K-ras activation. These results are discussed in the context of the ras-signalling pathway.

publication date

  • August 30, 1995

Research

keywords

  • Academic Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1006/bbrc.1995.2034

PubMed ID

  • 7626109

Additional Document Info

start page

  • 758

end page

  • 66

volume

  • 212

number

  • 3