Activation of Tsk and Btk tyrosine kinases by G protein βγ subunits Academic Article Article uri icon


MeSH Major

  • GTP-Binding Proteins
  • Gene Expression Regulation
  • Signal Transduction


  • Tsk/Itk and Btk are members of the pleckstrin-homology (PH) domain-containing tyrosine kinase family. The PH domain has been demonstrated to be able to interact with beta gamma subunits of heterotrimeric guanine nucleotide-binding proteins (G proteins) (G beta gamma) and phospholipids. Using cotransfection assays, we show here that the kinase activities of Tsk and Btk are stimulated by certain G beta gamma subunits. Furthermore, using an in vitro reconstitution assay with purified bovine brain G beta gamma subunits and the immunoprecipitated Tsk, we find that Tsk kinase activity is increased by G beta gamma subunits and another membrane factor(s). These results indicate that this family of tyrosine kinases could be an effector of heterotrimeric G proteins.

publication date

  • September 12, 1995



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1073/pnas.92.19.8601

PubMed ID

  • 7567982

Additional Document Info

start page

  • 8601

end page

  • 5


  • 92


  • 19