Identification of a binding site in the disintegrin domain of fertilin required for sperm-egg fusion Academic Article uri icon


MeSH Major

  • Membrane Fusion
  • Membrane Glycoproteins
  • Metalloendopeptidases
  • Oligopeptides
  • Oocytes
  • Peptides
  • Sperm-Ovum Interactions
  • Spermatozoa


  • Fertilization and certain later stages in mammalian embryonic development require fusion between membranes of individual cells. The mechanism of eukaryotic cell-cell fusion is unknown, and no surface molecules required for this process have been unequivocally identified. The role of the sperm surface protein fertilin in sperm-egg fusion was tested by using peptide analogues of a potential integrin binding site in the fertilin beta subunit. Peptide analogues that include a TDE sequence from the disintegrin region of fertilin beta are able to bind to the egg plasma membrane and strongly inhibit sperm-egg fusion. These results show that the disintegrin domain of fertilin beta binds to the egg plasma membrane and that this binding is required for membrane fusion.

publication date

  • May 10, 1994



  • Academic Article



  • eng

PubMed Central ID

  • PMC43751

PubMed ID

  • 8183890

Additional Document Info

start page

  • 4195

end page

  • 8


  • 91


  • 10