Applications of ratio fluorescence microscopy in the study of cell physiology Review uri icon

Overview

MeSH Major

  • Cell Physiological Phenomena
  • Microscopy, Fluorescence

abstract

  • Quantitative fluorescence microscopy is becoming an increasingly important tool in the study of cell biology. Fluorescence microscopy has long been used for qualitative characterizations of subcellular distributions of proteins, lipids, nucleic acids, and ions, but quantifying these distributions is complicated by a variety of optical, biological, and physical factors. Many factors that complicate quantification of fluorescence in cells can be circumvented by analyzing fluorescence ratios derived from pairs of fluorescence images. In this review we will discuss the factors that affect fluorescence quantification, the advantages of quantifying fluorescence as a ratio, and give examples of how fluorescence ratio microscopy is being applied in studies of cell biology.

publication date

  • July 8, 1994

Research

keywords

  • Review

Identity

Language

  • eng

PubMed ID

  • 8005385

Additional Document Info

start page

  • 573

end page

  • 82

volume

  • 8

number

  • 9