Gene therapy for brain tumors: Regression of experimental gliomas by adenovirus-mediated gene transfer in vivo Academic Article uri icon

Overview

MeSH Major

  • Brain Neoplasms
  • Genetic Therapy
  • Glioma

abstract

  • The therapeutic efficacy of adenovirus-mediated herpes simplex virus thymidine kinase (HSV-tk) gene transduction of rat C6 glioma cells followed by ganciclovir (GCV) administration was studied in tumors generated in the brains of nude mice. C6 glioma cells were efficiently transduced in vitro by a replicative-defective recombinant adenovirus carrying the HSV-tk gene (ADV/RSV-tk) that rendered them sensitive to GCV in a dose-dependent manner. Tumors were generated by stereotaxic intracerebral injection of 1 x 10(4) C6 cells in nude mice. After 8 days of tumor growth, 3 x 10(8) ADV/RSV-tk viral particles were injected into the tumors and the mice subsequently were treated with GCV for 6 days. Tumor size in untreated and treated animals was compared 20 days after tumor implantation. The mean cross-sectional area of the tumors in the treated animals was 23-fold smaller than in control animals and the tumor volume was reduced by > 500-fold. These results demonstrate that the recombinant adenoviral vector can function as an efficient gene delivery vehicle for the treatment of gliomas by in vivo gene therapy.

publication date

  • April 12, 1994

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC43513

PubMed ID

  • 8159705

Additional Document Info

start page

  • 3054

end page

  • 7

volume

  • 91

number

  • 8