Administration of an adenovirus containing the human CFTR cDNA to the respiratory tract of individuals with cystic fibrosis. Academic Article uri icon

Overview

MeSH

  • Adult
  • Base Sequence
  • Bronchi
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • DNA, Complementary
  • Epithelium
  • Female
  • Genetic Vectors
  • Humans
  • Interleukin-6
  • Lung
  • Male
  • Molecular Sequence Data
  • Nose

MeSH Major

  • Adenoviridae
  • Cystic Fibrosis
  • DNA, Recombinant
  • Genetic Therapy
  • Membrane Proteins
  • Respiratory System

abstract

  • We have administered a recombinant adenovirus vector (AdCFTR) containing the normal human CFTR cDNA to the nasal and bronchial epithelium of four individuals with cystic fibrosis (CF). We show that this vector can express the CFTR cDNA in the CF respiratory epithelium in vivo. With doses up to 2 x 10(9) pfu, there was no recombination/complementation or shedding of the vector or rise of neutralizing antibody titres. At 2 x 10(9) pfu, a transient systemic and pulmonary syndrome was observed, possibly mediated by interleukin-6. Follow-up at 6-12 months demonstrated no long term adverse effects. Thus, it is feasible to use an adenovirus vector to transfer and express the CFTR cDNA in the respiratory epithelium of individuals with CF. Correction of the CF phenotype of the airway epithelium might be achieved with this strategy.

publication date

  • September 1994

has subject area

  • Adenoviridae
  • Adult
  • Base Sequence
  • Bronchi
  • Cystic Fibrosis
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • DNA, Complementary
  • DNA, Recombinant
  • Epithelium
  • Female
  • Genetic Therapy
  • Genetic Vectors
  • Humans
  • Interleukin-6
  • Lung
  • Male
  • Membrane Proteins
  • Molecular Sequence Data
  • Nose
  • Respiratory System

Research

keywords

  • Clinical Trial
  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1038/ng0994-42

PubMed ID

  • 7527271

Additional Document Info

start page

  • 42

end page

  • 51

volume

  • 8

number

  • 1