The tumor protein MAGE-1 is located in the cytosol of human melanoma cells Academic Article Article uri icon

Overview

MeSH Major

  • Antigens, Neoplasm
  • Cancer Vaccines
  • Neoplasms
  • Testis

abstract

  • The MAGE-1 protein has been localized to the cytosol of human melanoma culture cells (MZ2-MEL.43) by subcellular fractionation. Its distribution between nuclear, mitochondrial, microsomal and cytosolic fractions was established by SDS-PAGE and immunoblotting with a rabbit antiserum and compared to that of markers for the main cell compartments. The immunoreactive material was recovered in the cytosolic fraction as a polypeptide migrating at 42 kDa, which has been further identified as the MAGE-1 protein because it comigrates with the product of cell-free transcription-translation of the MAGE-1 cDNA. Location to the cytosol was confirmed by immunofluorescence microscopy.

publication date

  • January 1994

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1006/bbrc.1994.2517

PubMed ID

  • 7980533

Additional Document Info

start page

  • 710

end page

  • 5

volume

  • 204

number

  • 2