PU.1 and an HLH family member contribute to the myeloid-specific transcription of the Fc gamma RIIIA promoter. Academic Article Article uri icon

Overview

MeSH

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes
  • Base Sequence
  • DNA-Binding Proteins
  • Fibroblasts
  • Helix-Loop-Helix Motifs
  • Mice
  • Molecular Sequence Data
  • Protein Binding
  • Receptors, IgG
  • Retroviridae Proteins, Oncogenic
  • Tissue Distribution
  • Tumor Cells, Cultured

MeSH Major

  • Macrophages
  • Promoter Regions, Genetic
  • Transcription Factors
  • Transcription, Genetic

abstract

  • Expression of the low-affinity Fc receptor for IgG (murine Fc gamma RIIIA) is restricted to cells of myelomonocytic origin. We report here the promoter structure, the proximal DNA sequences responsible for transcription of Fc gamma RIIIA in macrophages and the protein factors which interact with these sequences. A 51 bp sequence, termed the myeloid restricted region (MRR), was both necessary and sufficient for conferring cell type-specific expression in macrophages. Reporter constructs containing mutations in this sequence result in the loss of MRR activity upon transfection into the macrophage cell line, RAW264.7. Two cis-acting elements have been identified and are required for full promoter function. These same elements analyzed by EMSA define two binding sites recognized by nuclear factors derived from macrophages. A 3' purine tract (-50 to -39) within the MRR binds the macrophage and B cell-specific factor, PU.1, and a second E box-like element, termed MyE, upstream of the PU.1 box (-88 to -78) binds the HLH factors TFE3 and USF. EMSA studies using RAW cell extracts suggest that both PU.1 and MyE factors may bind simultaneously to the MRR resulting in a ternary complex that is responsible, in part, for the myeloid-specific activity of the Fc gamma RIIIA promoter.

publication date

  • August 15, 1994

has subject area

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes
  • Base Sequence
  • DNA-Binding Proteins
  • Fibroblasts
  • Helix-Loop-Helix Motifs
  • Macrophages
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Protein Binding
  • Receptors, IgG
  • Retroviridae Proteins, Oncogenic
  • Tissue Distribution
  • Transcription Factors
  • Transcription, Genetic
  • Tumor Cells, Cultured

Research

keywords

  • Comparative Study
  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC395298

PubMed ID

  • 8070412

Additional Document Info

start page

  • 3852

end page

  • 3860

volume

  • 13

number

  • 16