[Damage to calcium ion-loaded mitochondria by fatty acids and the protective effect of carnitine]. Academic Article uri icon

Overview

MeSH

  • Adenosine Diphosphate
  • Adenosine Triphosphate
  • Animals
  • Atractyloside
  • Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
  • Rats

MeSH Major

  • Calcium
  • Carnitine
  • Fatty Acids
  • Mitochondria, Liver

abstract

  • The effect of fatty acids and L-carnitine on Ca2+ retention in rat liver mitochondria have been studied. Ca(2+)-retention was estimated as a sum of consecutive Ca2+ additions which leaded to transient stimulation of respiration coupled with influx of Ca2+ L-carnitine increases the Ca(2+)-retention; such an effect requires ATP. The Ca(2+)-retention was increased in the presence of 50 microM ATP or ADP. In all cases carboxyatractylate prevented the increase in Ca(2+)-retention. Palmitate and FCCP added at concentrations producing similar stimulating effect on respiration inhibit Ca(2+)-retention to about the same degree. The effect of palmitate is strongly diminished by L-carnitine. Again, the L-carnitine effect requires ATP. The data obtained suggest that the protonophoric effect of fatty acid plays a crucial role in Ca(2+)-dependent damage of mitochondria.

publication date

  • April 1993

has subject area

  • Adenosine Diphosphate
  • Adenosine Triphosphate
  • Animals
  • Atractyloside
  • Calcium
  • Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
  • Carnitine
  • Fatty Acids
  • Mitochondria, Liver
  • Rats

Research

keywords

  • Journal Article

Identity

Language

  • rus

PubMed ID

  • 8507734

Additional Document Info

start page

  • 585

end page

  • 589

volume

  • 58

number

  • 4