Efficient gene transfer into myocardium by direct injection of adenovirus vectors. Academic Article uri icon

Overview

MeSH

  • Animals
  • Gene Expression
  • Injections
  • Rats
  • Rats, Sprague-Dawley
  • beta-Galactosidase

MeSH Major

  • Adenoviridae
  • Gene Transfer Techniques
  • Genetic Vectors
  • Myocardium

abstract

  • Previous studies have established that gene transfer into myocardial cells in vivo is detectable after direct injection of plasmid DNA. Recently, adenovirus vectors have been shown to provide an efficient method for gene transfer into a wide range of tissues. Therefore, this study sought to assess the efficiency and stability of adenovirus-mediated gene transfer into myocardium and to compare this method with that using plasmid-based gene transfer techniques. Adult rats underwent myocardial injection via a subdiaphragmatic approach. Gene transfer efficiency was compared using direct injection of an adenovirus vector encoding for the marker gene beta-galactosidase (beta-gal), a control adenovirus vector encoding for the cystic fibrosis transmembrane conductance regulator gene, a plasmid encoding for beta-gal, or a control plasmid. Hearts infected with an adenovirus vector containing the beta-gal gene showed significantly increased beta-gal enzymatic activity compared with hearts injected with beta-gal plasmid. Histological examination revealed that cardiac myocytes were the target of adenovirus-mediated gene transfer. A time course of gene expression showed that beta-gal enzymatic activity peaked during the first week following injection. Adenovirus vectors provide an efficient but transient method for in vivo gene expression in myocardium.

publication date

  • December 1993

has subject area

  • Adenoviridae
  • Animals
  • Gene Expression
  • Gene Transfer Techniques
  • Genetic Vectors
  • Injections
  • Myocardium
  • Rats
  • Rats, Sprague-Dawley
  • beta-Galactosidase

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 8222091

Additional Document Info

start page

  • 1202

end page

  • 1207

volume

  • 73

number

  • 6