Resolution enhanced NMR spectroscopy in biological systems via magnetic susceptibility matched sample immersion chambers Academic Article uri icon


MeSH Major

  • Arousal
  • Corpus Striatum
  • Deep Brain Stimulation
  • Frontal Lobe
  • Psychomotor Performance
  • Thalamus


  • A technique is described which reduced the magnetic susceptibility induced line broadening in NMR spectra obtained from three biological systems at 4.7 Tesla. Proton spectra from a sealed suspension of HL60 leukemic myeloblasts yielded minimum linewidths of 1.3 Hz at 200 MHz (0.0065 ppm) after 10 min of automated shimming. 31P spectra from an in vivo murine MCa mammary carcinoma yielded a well-resolved phosphorylcholine resonance without proton decoupling and with the resistive shim coil currents set to zero. 31P spectra from a perfused suspension of RIF-1 fibrosarcoma cells exhibited a gamma-nucleoside triphosphate resonance which was resolved into purine and pyrimidine components.

publication date

  • December 17, 1993



  • Academic Article


Digital Object Identifier (DOI)

  • 10.1002/mrm.1910300615

Additional Document Info

start page

  • 754

end page

  • 8


  • 30


  • 6