Resolution enhanced NMR spectroscopy in biological systems via magnetic susceptibility matched sample immersion chambers. Academic Article uri icon

Overview

MeSH

  • Animals
  • Fibrosarcoma
  • Leukemia, Experimental
  • Mammary Neoplasms, Experimental
  • Mice
  • Mice, Inbred C3H
  • Tumor Cells, Cultured

MeSH Major

  • Magnetic Resonance Spectroscopy

abstract

  • A technique is described which reduced the magnetic susceptibility induced line broadening in NMR spectra obtained from three biological systems at 4.7 Tesla. Proton spectra from a sealed suspension of HL60 leukemic myeloblasts yielded minimum linewidths of 1.3 Hz at 200 MHz (0.0065 ppm) after 10 min of automated shimming. 31P spectra from an in vivo murine MCa mammary carcinoma yielded a well-resolved phosphorylcholine resonance without proton decoupling and with the resistive shim coil currents set to zero. 31P spectra from a perfused suspension of RIF-1 fibrosarcoma cells exhibited a gamma-nucleoside triphosphate resonance which was resolved into purine and pyrimidine components.

publication date

  • December 1993

has subject area

  • Animals
  • Fibrosarcoma
  • Leukemia, Experimental
  • Magnetic Resonance Spectroscopy
  • Mammary Neoplasms, Experimental
  • Mice
  • Mice, Inbred C3H
  • Tumor Cells, Cultured

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 8139459

Additional Document Info

start page

  • 754

end page

  • 758

volume

  • 30

number

  • 6