Inhibition of viral replication by interferon-γ-induced nitric oxide synthase Academic Article uri icon


MeSH Major

  • Amino Acid Oxidoreductases
  • Ectromelia virus
  • Interferon-gamma
  • Macrophages
  • Virus Replication


  • Interferons (IFNs) induce antiviral activity in many cell types. The ability of IFN-gamma to inhibit replication of ectromelia, vaccinia, and herpes simplex-1 viruses in mouse macrophages correlated with the cells' production of nitric oxide (NO). Viral replication was restored in IFN-gamma-treated macrophages exposed to inhibitors of NO synthase. Conversely, epithelial cells with no detectable NO synthesis restricted viral replication when transfected with a complementary DNA encoding inducible NO synthase or treated with organic compounds that generate NO. In mice, an inhibitor of NO synthase converted resolving ectromelia virus infection into fulminant mousepox. Thus, induction of NO synthase can be necessary and sufficient for a substantial antiviral effect of IFN-gamma.

publication date

  • January 1993



  • Academic Article



  • eng

PubMed ID

  • 7690156

Additional Document Info

start page

  • 1445

end page

  • 8


  • 261


  • 5127