Activation of the sphingomyelin signaling pathway in intact EL4 cells and in a cell-free system by IL-1β Academic Article uri icon

Overview

MeSH Major

  • Ceramides
  • Interleukin-1
  • Signal Transduction
  • Sphingomyelins

abstract

  • The mechanism of interleukin-1 (IL-1) signaling is unknown. Tumor necrosis factor-alpha uses a signal transduction pathway that involves sphingomyelin hydrolysis to ceramide and stimulation of a ceramide-activated protein kinase. In intact EL4 thymoma cells, IL-1 beta similarly stimulated a rapid decrease of sphingomyelin and an elevation of ceramide, and enhanced ceramide-activated protein kinase activity. This cascade was also activated by IL-1 beta in a cell-free system, demonstrating tight coupling to the receptor. Exogenous sphingomyelinase, but not phospholipases A2, C, or D, in combination with phorbol ester replaced IL-1 beta to stimulate IL-2 secretion. Thus, IL-1 beta signals through the sphingomyelin pathway.

publication date

  • January 22, 1993

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 8424175

Additional Document Info

start page

  • 519

end page

  • 22

volume

  • 259

number

  • 5094