Circulating borrelia burgdorferi in patients with acute Lyme disease: Results of blood cultures and serum DNA analysis Academic Article uri icon


MeSH Major

  • Borrelia burgdorferi Group
  • Lyme Disease


  • To determine the usefulness of blood culture and polymerase chain reaction (PCR) analysis in detecting circulating Borrelia burgdorferi or its DNA, blood and serum from untreated patients with acute Lyme disease were examined. None of the cultures of blood or serum from the 7 patients tested demonstrated spirochetes. Similarly, all patient serum samples, assayed in two laboratories, were negative for B. burgdorferi DNA using PCR amplification. These results suggest that in patients with acute Lyme disease, spirochetes, spirochete DNA, or both circulate early, only intermittently, or at low levels and that neither culture nor PCR testing of blood or serum, as currently done, appears likely to prove generally useful in the diagnosis of Lyme disease.

publication date

  • December 1993



  • Academic Article



  • eng

PubMed ID

  • 8018136

Additional Document Info

start page

  • 1541

end page

  • 3


  • 168


  • 6