Serial phenotypic, cytogenetic and molecular genetic studies in Richter's syndrome: demonstration of lymphoma development from the chronic lymphocytic leukaemia cells Academic Article Article uri icon

Overview

MeSH Major

  • Disease Outbreaks
  • Mycobacterium Infections, Nontuberculous
  • Skin Diseases, Bacterial
  • Soft Tissue Infections

abstract

  • In this report we describe a unique longitudinal study on the clinical, phenotypic, cytogenetic and molecular genetic features of malignant cells from diagnosis of chronic lymphocytic leukaemia (CLL) to the development of lymphoma and lymphomatous meningitis. CLL cells at diagnosis were CD5+, CD19+, surface IgG+, kappa+, were karyotypically abnormal and showed clonal rearrangements in the immunoglobulin heavy (IgH) and kappa light chain genes. Phenotypically leukaemic cells and lymphoma cells at RS resembled CLL at diagnosis, but showed cytogenetic evolution. Geometrically leukaemic cells and lymphoma cells retained the initial clonal rearrangements in IGH and kappa genes, but showed additional supervening clonal rearrangements in both of these genes as the disease progressed to RS. Furthermore, the c-lambda DNA showed clonal rearrangements in the leukaemic cells and lymphoma cells at RS. This complete phenotypic and genotypic analysis of tumour cells during the course of the disease demonstrates the origin of lymphoma from CLL cells through progressive cytogenetic and molecular genetic changes in CLL cells.

publication date

  • January 1993

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1111/j.1365-2141.1993.tb03357.x

PubMed ID

  • 8136284

Additional Document Info

start page

  • 613

end page

  • 6

volume

  • 85

number

  • 3