Detection of immunoglobulin gene rearrangement of B cell nonHodgkin's lymphomas and leukemias in fresh, unfixed and formalin-fixed, paraffin- embedded tissue by polymerase chain reaction
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Immunoglobulin Variable Region
Our results suggest that the monoclonality of human neoplastic B cells can be efficiently evaluated by PCR equally well from fresh, unfixed and formalin-fixed, paraffin-embedded tissues. This technique should prove to be a powerful tool in clinical diagnosis and research as well as in the retrospective analysis of archival pathologic specimens.