Activation of alveolar macrophages in asymptomatic HIV-infected individuals. Academic Article uri icon

Overview

MeSH

  • Adult
  • Bronchoalveolar Lavage Fluid
  • Cells, Cultured
  • Chemokine CXCL10
  • Cytokines
  • Female
  • Histocompatibility Antigens Class II
  • Humans
  • Interferon-gamma
  • Male
  • RNA, Messenger
  • Superoxides
  • Tumor Necrosis Factor-alpha

MeSH Major

  • Chemokines, CXC
  • HIV Infections
  • Macrophage Activation
  • Macrophages, Alveolar

abstract

  • After the initial infection with HIV, there is evidence of immune dysfunction despite an apparent normal clinical state. In the context that the lung is a major site affected by opportunistic infection during the progression of this immune dysfunction, and that some components of the immune system are activated during early HIV infection, we hypothesized that there may be activation of alveolar macrophages (AM), a key component of the pulmonary host defense system, during the asymptomatic phase of HIV infection. Compared to normals, in HIV-infected individuals the class II MHC molecules DR, DQ, and DP were all expressed more frequently and in greater cell surface density on AM (p < 0.03, all comparisons), and there was increased spontaneous release of superoxide anion (O2-.) by AM (p < 0.002). To gain insight into whether the activation of the AM was an inherent property of the cells or dependent on the in vivo milieu, AM were evaluated after 24 h in culture for O2-. release. In contrast to the findings in fresh AM, after 24 h in culture, O2-. release by HIV AM was not different from normals (p > 0.7), suggesting that these AM had been activated in vivo. To assess whether IFN-gamma could be mediating these effects, mRNA levels of the IP-10 gene (a gene specifically induced by increased concentrations of IFN-gamma) were quantified in AM. Strikingly, the IP-10 gene was expressed only in AM of HIV-seropositive individuals, suggesting the AM had been exposed to IFN-gamma in vivo. Overall, these observations are consistent with the concept that the HIV-seropositive state is associated with activation of AM, in part due to local exposure to IFN-gamma.

publication date

  • February 1, 1993

has subject area

  • Adult
  • Bronchoalveolar Lavage Fluid
  • Cells, Cultured
  • Chemokine CXCL10
  • Chemokines, CXC
  • Cytokines
  • Female
  • HIV Infections
  • Histocompatibility Antigens Class II
  • Humans
  • Interferon-gamma
  • Macrophage Activation
  • Macrophages, Alveolar
  • Male
  • RNA, Messenger
  • Superoxides
  • Tumor Necrosis Factor-alpha

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 8380824

Additional Document Info

start page

  • 1019

end page

  • 1028

volume

  • 150

number

  • 3