Cloning and analysis of the locus for mosquito vitellogenic carboxypeptidase. Academic Article uri icon

Overview

MeSH

  • Animals
  • Base Sequence
  • Binding Sites
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA
  • Fat Body
  • Female
  • Molecular Sequence Data
  • Peptide Chain Initiation, Translational
  • Protein Binding
  • Rats

MeSH Major

  • Aedes
  • Carboxypeptidases
  • Insect Proteins

abstract

  • Vitellogenic carboxypeptidase is a 53 kDa yolk protein produced by the fat body of the female mosquito, Aedes aegypti, in response to a blood meal. Its expression is sex-, stage- and tissue-specific and is identical to that of the major yolk protein, vitellogenin. The gene is intronless and two alleles have been cloned and sequenced, including more than 1.5 kb on both sides of the coding region. A capsite consensus recently identified as an arthropod initiator is present at the start site of transcription. Upstream of this capsite is a 16 bp imperfect palindrome repeated four times showing strong homology to defined hormone-response elements. In addition, a region that closely resembles the fat body enhancer and double sex binding site from the Drosophila yolk protein genes and several potential fat body-specific regulatory protein binding sites were found.

publication date

  • 1993

has subject area

  • Aedes
  • Animals
  • Base Sequence
  • Binding Sites
  • Carboxypeptidases
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA
  • Fat Body
  • Female
  • Insect Proteins
  • Molecular Sequence Data
  • Peptide Chain Initiation, Translational
  • Protein Binding
  • Rats

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 9087558

Additional Document Info

start page

  • 205

end page

  • 213

volume

  • 2

number

  • 4