Adenovirus-mediated in vivo gene transfer and expression in normal rat liver. Academic Article uri icon

Overview

MeSH

  • Animals
  • Cells, Cultured
  • DNA
  • Escherichia coli
  • Genetic Vectors
  • Humans
  • RNA, Messenger
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Fusion Proteins
  • beta-Galactosidase

MeSH Major

  • Adenoviruses, Human
  • Liver
  • Transfection
  • alpha 1-Antitrypsin

abstract

  • Replication deficient, recombinant adenovirus (Ad) vectors do not require target cell replication for transfer and expression of exogenous genes and thus may be useful for in vivo gene therapy in hepatocytes. In vitro, primary cultures of rat hepatocytes infected with a recombinant Ad containing a human alpha 1-antitrypsin cDNA (Ad-alpha 1AT) synthesized and secreted human alpha 1AT for 4 weeks. In rats, in vivo intraportal administration of a recombinant Ad containing the E. coli lacZ gene, was followed by expression of beta-galactosidase in hepatocytes 3 days after infection. Intraportal infusion of Ad-alpha 1AT produced detectable serum levels of human alpha 1AT for 4 weeks. Thus, targeted gene expression has been achieved in the liver, albeit at low levels, suggesting that adenovirus vectors may be a useful means for in vivo gene therapy in liver disorders.

publication date

  • August 1992

has subject area

  • Adenoviruses, Human
  • Animals
  • Cells, Cultured
  • DNA
  • Escherichia coli
  • Genetic Vectors
  • Humans
  • Liver
  • RNA, Messenger
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Fusion Proteins
  • Transfection
  • alpha 1-Antitrypsin
  • beta-Galactosidase

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1038/ng0892-372

PubMed ID

  • 1302034

Additional Document Info

start page

  • 372

end page

  • 378

volume

  • 1

number

  • 5