Production of the Alzheimer amyloid β protein by normal proteolytic processing Academic Article uri icon

Overview

MeSH Major

  • Alzheimer Disease
  • Amyloid beta-Peptides
  • Leukemia, Myeloid
  • Neuroblastoma

abstract

  • The 4-kilodalton (39 to 43 amino acids) amyloid beta protein (beta AP), which is deposited as amyloid in the brains of patients with Alzheimer's diseases, is derived from a large protein, the amyloid beta protein precursor (beta APP). Human mononuclear leukemic (K562) cells expressing a beta AP-bearing, carboxyl-terminal beta APP derivative released significant amounts of a soluble 4-kilodalton beta APP derivative essentially identical to the beta AP deposited in Alzheimer's disease. Human neuroblastoma (M17) cells transfected with constructs expressing full-length beta APP and M17 cells expressing only endogenous beta APP also released soluble 4-kilodalton beta AP, and a similar, if not identical, fragment was readily detected in cerebrospinal fluid from individuals with Alzheimer's disease and normal individuals. Thus cells normally produce and release soluble 4-kilodalton beta AP that is essentially identical to the 4-kilodalton beta AP deposited as insoluble amyloid fibrils in Alzheimer's disease.

publication date

  • December 1992

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 1439760

Additional Document Info

start page

  • 126

end page

  • 9

volume

  • 258

number

  • 5079