Influenza virus A infection induces interleukin-8 gene expression in human airway epithelial cells. Academic Article uri icon

Overview

MeSH

  • Base Sequence
  • Cells, Cultured
  • DNA
  • Epithelial Cells
  • Epithelium
  • Humans
  • Molecular Sequence Data
  • Virus Replication

MeSH Major

  • Gene Expression Regulation
  • Influenza A virus
  • Interleukin-8
  • Lung

abstract

  • To determine the role of the airway epithelial cell in mediating virus-induced inflammation, we infected primary cultures of human airway epithelial cells with human influenza type A/Port Chalmers/72 (H3N2). After two days, the medium was collected for measurement of the chemotactic cytokine interleukin-8 by enzyme-linked immunosorbent assay. The RNA was extracted from the cells for analysis of interleukin-8 mRNA by Northern blot analysis. Interleukin-8 production was more than doubled by viral infection, while interleukin-8 mRNA was increased four-fold. Thus induction of interleukin-8 gene expression in virus-infected airway epithelium may be an important early step leading to virus-induced airway inflammation.

publication date

  • September 14, 1992

has subject area

  • Base Sequence
  • Cells, Cultured
  • DNA
  • Epithelial Cells
  • Epithelium
  • Gene Expression Regulation
  • Humans
  • Influenza A virus
  • Interleukin-8
  • Lung
  • Molecular Sequence Data
  • Virus Replication

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 1516705

Additional Document Info

start page

  • 327

end page

  • 329

volume

  • 309

number

  • 3