Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme Academic Article uri icon

Overview

MeSH Major

  • Amino Acid Oxidoreductases
  • Brain
  • Isoenzymes
  • Macrophages
  • Neurons

abstract

  • Nitric oxide (NO) is a messenger molecule of macrophages, endothelial cells in blood vessels, and neurons. A neuronal form of NO synthase (NOS) has been previously cloned. We now report the molecular cloning of macrophage NOS. The macrophage enzyme displays 50% sequence identity to the neuronal enzyme. Like neuronal NOS, macrophage NOS has recognition sites for FAD, FMN, and NADPH and also has a consensus calmodulin binding site. Macrophage NOS mRNA is strikingly inducible; it is absent in quiescent macrophages or spleen but is prominent 2-6 hr after endotoxin treatment.

publication date

  • December 1992

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC49573

PubMed ID

  • 1379716

Additional Document Info

start page

  • 6711

end page

  • 5

volume

  • 89

number

  • 15