Carbodiimide modification reduces the conductance and increases the tetrodotoxin sensitivity in batrachotoxin-modified sodium channels Academic Article uri icon

Overview

MeSH Major

  • Batrachotoxins
  • CME-Carbodiimide
  • Carbodiimides
  • Neural Conduction
  • Sodium Channels
  • Tetrodotoxin

abstract

  • The relationship between the channel entrance and the tetrodotoxin (TTX) binding site was investigated by chemical modification at the extracellular surface of bilayer-incorporated batrachotoxin-(BTX) modified sodium channels using an impermeant carbodiimide in the presence or absence of exogenous nucleophiles. Two (classes of) groups could be modified such that the open-channel conductance was decreased while TTX binding was unaffected, and TTX did not protect against this modification. Because the final conductance level depends on the exogenous nucleophile, each covalent modification appears to involve a carboxyl group. In addition, a third (carboxyl) group could be modified such that TTX binding affinity was increased. These results suggest that the channel entrance and the TTX binding site are spatially separate, which supports previous suggestions that the mechanism by which guanidinium toxins close sodium channels involves a conformational change subsequent to toxin binding.

publication date

  • August 14, 1992

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 1326748

Additional Document Info

start page

  • 262

end page

  • 9

volume

  • 421

number

  • 2-3