Cloning and characterization of inducible nitric oxide synthase from mouse macrophages Academic Article uri icon


MeSH Major

  • Amino Acid Oxidoreductases
  • Isoenzymes
  • Macrophages


  • Nitric oxide (NO) conveys a variety of messages between cells, including signals for vasorelaxation, neurotransmission, and cytotoxicity. In some endothelial cells and neurons, a constitutive NO synthase is activated transiently by agonists that elevate intracellular calcium concentrations and promote the binding of calmodulin. In contrast, in macrophages, NO synthase activity appears slowly after exposure of the cells to cytokines and bacterial products, is sustained, and functions independently of calcium and calmodulin. A monospecific antibody was used to clone complementary DNA that encoded two isoforms of NO synthase from immunologically activated mouse macrophages. Liquid chromatography-mass spectrometry was used to confirm most of the amino acid sequence. Macrophage NO synthase differs extensively from cerebellar NO synthase. The macrophage enzyme is immunologically induced at the transcriptional level and closely resembles the enzyme in cytokine-treated tumor cells and inflammatory neutrophils.

publication date

  • January 1992



  • Academic Article



  • eng

PubMed ID

  • 1373522

Additional Document Info

start page

  • 225

end page

  • 8


  • 256


  • 5054