In vivo transfer of the human cystic fibrosis transmembrane conductance regulator gene to the airway epithelium. Academic Article uri icon

Overview

MeSH

  • Adenoviruses, Human
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cell Line
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • DNA
  • Genetic Therapy
  • Genetic Vectors
  • Humans
  • Immunohistochemistry
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction
  • RNA, Messenger
  • Sigmodontinae
  • Transcription, Genetic

MeSH Major

  • Cystic Fibrosis
  • Lung
  • Membrane Proteins
  • Transfection

abstract

  • Direct transfer of the normal cystic fibrosis (CF) transmembrane conductance regulator (CFTR) gene to airway epithelium was evaluated using a replication-deficient recombinant adenovirus (Ad) vector containing normal human CFTR cDNA (Ad-CFTR). In vitro Ad-CFTR-infected CFPAC-1 CF epithelial cells expressed human CFTR mRNA and protein and demonstrated correction of defective cAMP-mediated Cl- permeability. Two days after in vivo intratracheal introduction of Ad-CFTR in cotton rats, in situ analysis demonstrated human CFTR gene expression in lung epithelium. PCR amplification of reverse transcribed lung RNA demonstrated human CFTR transcripts derived from Ad-CFTR, and Northern analysis of lung RNA revealed human CFTR transcripts for up to 6 weeks. Human CFTR protein was detected in epithelial cells using anti-human CFTR antibody 11-14 days after infection. While the safety and effectiveness remain to be demonstrated, these observations suggest the feasibility of in vivo CFTR gene transfer as therapy for the pulmonary manifestations of CF.

publication date

  • January 10, 1992

has subject area

  • Adenoviruses, Human
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cell Line
  • Cystic Fibrosis
  • Cystic Fibrosis Transmembrane Conductance Regulator
  • DNA
  • Genetic Therapy
  • Genetic Vectors
  • Humans
  • Immunohistochemistry
  • Lung
  • Membrane Proteins
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction
  • RNA, Messenger
  • Sigmodontinae
  • Transcription, Genetic
  • Transfection

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 1370653

Additional Document Info

start page

  • 143

end page

  • 155

volume

  • 68

number

  • 1