Thrombospondin sequence motif (CSVTCG) is responsible for CD36 binding Academic Article Article uri icon

Overview

MeSH Major

  • Ethnic Groups
  • Malaria
  • Qualitative Research
  • Residence Characteristics
  • Social Marginalization

abstract

  • To clarify the role of CD36 as a TSP receptor and to investigate the mechanisms of the TSP-CD36 interaction, transfection studies were performed using CD36-cDNA in a CDM8 plasmid. Jurkat cells transfected with CD36 cDNA express an 88kD membrane surface protein and acquire the ability to bind thrombospondin. The TSP amino acid sequence, CSVTCG, mediates the interaction of thrombospondin with CD36. CD36 transfectants but not control transfectants bind radiolabeled tyrosinated peptide (YCSVTCG). The hexapeptide inhibits thrombospondin expression on activated human platelets and results in diminished platelet aggregation. CSVTCG-albumin conjugates support CD36-dependent adhesion of tumor cells. We conclude that the CSVTCG repeat sequence is a crucial determinant of CD36 thrombospondin binding.

publication date

  • February 14, 1992

Research

keywords

  • Academic Article

Identity

Digital Object Identifier (DOI)

  • 10.1016/0006-291X(92)91860-S

PubMed ID

  • 1371676

Additional Document Info

start page

  • 1208

end page

  • 17

volume

  • 182

number

  • 3