Inhibition of neutrophil chemokinesis on vitronectin by inhibitors of calcineurin Academic Article uri icon

Overview

MeSH Major

  • Calmodulin-Binding Proteins
  • Chemotaxis, Leukocyte
  • Glycoproteins
  • Neutrophils
  • Phosphoprotein Phosphatases

abstract

  • Migration of human polymorphonuclear neutrophils on vitronectin is dependent on repeated transient increases in the concentration of intracellular free calcium ([Ca2+]i). A specific peptide inhibitor of the Ca(2+)-calmodulin-dependent phosphatase calcineurin was introduced into the cytoplasm of neutrophils. The peptide inhibited neutrophil migration on vitronectin by interfering with the release of the cells from sites of attachment. A similar reduction in motility on vitronectin occurred when cells were treated with the immunosuppressant FK506, which also inhibits calcineurin when bound to its binding protein, FKBP. These results indicate that a rise in [Ca2+]i reduces integrin-mediated adhesion to vitronectin by a mechanism that requires calcineurin activity.

publication date

  • October 9, 1992

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed ID

  • 1384129

Additional Document Info

start page

  • 296

end page

  • 9

volume

  • 258

number

  • 5080