Structure and promoter activity of the gene for the erythroid transcription factor GATA-1 Academic Article uri icon

Overview

MeSH Major

  • DNA-Binding Proteins
  • Erythrocytes
  • Gene Expression Regulation
  • Promoter Regions, Genetic
  • Regulatory Sequences, Nucleic Acid
  • Transcription Factors

abstract

  • We have cloned the gene for the chicken erythroid transcription factor GATA-1 (formerly Eryf1, NF-E1, or GF-1). The gene is composed of six exons, two of which encode the two finger domains of the protein. Transcription of GATA-1 in chicken embryonic erythroid cells initiates from multiple sites clustered approximately 200 base pairs upstream from the start of protein-coding sequence. A number of sequence motifs for known DNA-binding proteins are found to be protected in DNase I-footprinting experiments by either erythroid or brain nuclear extracts or by both. Notably, a cluster of three GATA-1 sites is protected by the erythroid extract, as well as by purified GATA-1. We find that the upstream region of the gene functions as a powerful promoter when transfected into embryonic erythroid cells. In primary chicken embryo fibroblasts the promoter exhibits lower activity, which is increased when the cells are cotransfected with a second plasmid expressing the GATA-1 cDNA. The results suggest that GATA-1 protein plays an autoregulatory role in its own expression.

publication date

  • December 1991

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC51372

PubMed ID

  • 2014222

Additional Document Info

start page

  • 3004

end page

  • 8

volume

  • 88

number

  • 8