Characterization of the promoter of the human gene encoding the high-affinity IgG receptor: transcriptional induction by gamma-interferon is mediated through common DNA response elements. Academic Article Article uri icon

Overview

MeSH

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cosmids
  • Female
  • HeLa Cells
  • Humans
  • Immunoglobulin G
  • Mice
  • Mice, Inbred A
  • Molecular Sequence Data
  • Placenta
  • Plasmids
  • Pregnancy
  • Receptors, IgG
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transfection

MeSH Major

  • Antigens, Differentiation
  • Genes
  • Interferon-gamma
  • Promoter Regions, Genetic
  • Receptors, Fc
  • Transcription, Genetic

abstract

  • Expression of the high-affinity receptor for IgG (Fc gamma RI) is restricted to cells of myeloid lineage and is induced by gamma-interferon (IFN-gamma) but not by IFN-alpha/beta. The organization of the human Fc gamma RI gene has been determined and the DNA elements governing its cell type-restricted transcription and IFN-gamma induction are reported here. A 39-nucleotide sequence (IFN-gamma response region, or GRR) is defined that is both necessary and sufficient for IFN-gamma inducibility. Sequence analysis of the GRR reveals the presence of promoter elements initially defined for the major histocompatibility complex class II genes: i.e., X, H, and gamma-IRE sequences. Comparison of a number of genes whose expression is induced selectively by IFN-gamma indicates that the presence of these elements is a general feature of IFN-gamma-responsive genes. Our studies suggest that the combination of X, H, and gamma-IRE elements is a common motif in the pathway of transcriptional induction by this lymphokine.

publication date

  • December 15, 1991

has subject area

  • Amino Acid Sequence
  • Animals
  • Antigens, Differentiation
  • Base Sequence
  • Cell Line
  • Cosmids
  • Female
  • Genes
  • HeLa Cells
  • Humans
  • Immunoglobulin G
  • Interferon-gamma
  • Mice
  • Mice, Inbred A
  • Molecular Sequence Data
  • Placenta
  • Plasmids
  • Pregnancy
  • Promoter Regions, Genetic
  • Receptors, Fc
  • Receptors, IgG
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic
  • Transfection

Research

keywords

  • Comparative Study
  • Journal Article

Identity

Language

  • eng

PubMed Central ID

  • PMC53123

PubMed ID

  • 1837149

Additional Document Info

start page

  • 11305

end page

  • 11309

volume

  • 88

number

  • 24