Preferential usage of the T-cell antigen receptor beta-chain constant region C beta 1 element by lung T-lymphocytes of patients with pulmonary sarcoidosis. Academic Article uri icon

Overview

MeSH

  • Adult
  • Female
  • Humans
  • Lung
  • Male
  • RNA Probes
  • RNA, Messenger

MeSH Major

  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Lung Diseases
  • Sarcoidosis
  • T-Lymphocytes

abstract

  • Evaluation of the T-cells accumulating at sites of disease in active sarcoidosis suggests the accumulation process is not random, evidenced by a bias in the types of T-cells present. To evaluate the concept that this bias extends to the accumulation of T-cells with the preferential use of specific T-cell antigen receptor (TCR) beta-chain constant region elements, beta-chain mRNA transcripts of lung and blood T-cells of normal subjects and patients with pulmonary sarcoidosis were compared for the relative usage of constant region beta 1 or beta 2 elements. Quantitative evaluation of C beta 1 and C beta 2 mRNA transcripts demonstrated a C beta 1/C beta 2 usage in normal blood of 0.63 +/- 0.02, similar to that of normal lung (0.64 +/- 0.06, p greater than 0.7), and in sarcoid blood (0.59 +/- 0.03, p greater than 0.2). In contrast, the lung T-lymphocytes of patients with sarcoidosis reflected a marked bias in the usage of C beta 1 elements (C beta 1/C beta 2: 0.88 +/- 0.06, p less than 0.001 compared with sarcoid blood and normal blood; p less than 0.02 compared with normal lung). Interestingly, a subgroup of these patients (six of 18) showed a markedly exaggerated skewing in the use of C beta 1 elements (C beta 1/C beta 2 ratio greater than 1, i.e., greater than 3 standard deviations above mean), demonstrating heterogeneity among sarcoid patients with regard to specific C beta 1 usage.(ABSTRACT TRUNCATED AT 250 WORDS)

publication date

  • March 1991

has subject area

  • Adult
  • Female
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Humans
  • Lung
  • Lung Diseases
  • Male
  • RNA Probes
  • RNA, Messenger
  • Sarcoidosis
  • T-Lymphocytes

Research

keywords

  • Journal Article

Identity

Language

  • eng

Digital Object Identifier (DOI)

  • 10.1164/ajrccm/143.3.635

PubMed ID

  • 1848052

Additional Document Info

start page

  • 635

end page

  • 639

volume

  • 143

number

  • 3