Immunohistochemical localization of granzyme B antigen in cytotoxic cells in human tissues Academic Article uri icon

Overview

MeSH Major

  • Antigens
  • Killer Cells, Natural
  • Serine Endopeptidases
  • T-Lymphocytes, Cytotoxic

abstract

  • Human granzyme B antigen is expressed in cytoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme B was generated using a prokaryotic expression vector under the control of T7 transcription and translation signals. The 25-kd recombinant protein (granzyme B) was used to develop a rabbit polyclonal antiserum. Purified anti-granzyme B antibodies were used to detect the antigen expression in cytotoxic cells in human tissues. Using the avidin-biotin-complex peroxidase technique, formalin-fixed, paraffin-embedded tissue sections from patients with acute mild or moderate allograft cardiac rejection were stained. A constant cytoplasmic staining of the lymphocytic allograft infiltrate was observed. These results provide a basis for using the anti-granzyme B antibodies for detection of cytotoxic cells in human tissues. The detection and quantitative analysis of the granzyme-associated cytotoxic cells may help to evaluate the significance of these functionally distinct cytotoxic cells in human tissues associated with increased expression of cytoplasmic granule effector molecules.

publication date

  • May 1991

Research

keywords

  • Academic Article

Identity

Language

  • eng

PubMed Central ID

  • PMC1886029

PubMed ID

  • 2024702

Additional Document Info

start page

  • 1069

end page

  • 75

volume

  • 138

number

  • 5