Adenovirus-mediated transfer of a recombinant alpha 1-antitrypsin gene to the lung epithelium in vivo. Academic Article uri icon

Overview

MeSH

  • Animals
  • Bronchi
  • Cystic Fibrosis
  • Emphysema
  • Epithelium
  • Gene Expression
  • Genetic Therapy
  • Humans
  • Promoter Regions, Genetic
  • RNA, Messenger
  • Sigmodontinae
  • Trachea
  • Transcription, Genetic
  • Virus Replication

MeSH Major

  • Adenoviridae
  • DNA, Recombinant
  • Genetic Vectors
  • Lung
  • Transfection
  • alpha 1-Antitrypsin

abstract

  • The respiratory epithelium is a potential site for somatic gene therapy for the common hereditary disorders alpha 1-antitrypsin (alpha 1AT) deficiency and cystic fibrosis. A replication-deficient adenoviral vector (Ad-alpha 1AT) containing an adenovirus major late promoter and a recombinant human alpha 1AT gene was used to infect epithelial cells of the cotton rat respiratory tract in vitro and in vivo. Freshly isolated tracheobronchial epithelial cells infected with Ad-alpha 1AT contained human alpha 1AT messenger RNA transcripts and synthesized and secreted human alpha 1AT. After in vivo intratracheal administration of Ad-alpha 1AT to these rats, human alpha 1AT messenger RNA was observed in the respiratory epithelium, human alpha 1AT was synthesized and secreted by lung tissue, and human alpha 1AT was detected in the epithelial lining fluid for at least 1 week.

publication date

  • April 19, 1991

has subject area

  • Adenoviridae
  • Animals
  • Bronchi
  • Cystic Fibrosis
  • DNA, Recombinant
  • Emphysema
  • Epithelium
  • Gene Expression
  • Genetic Therapy
  • Genetic Vectors
  • Humans
  • Lung
  • Promoter Regions, Genetic
  • RNA, Messenger
  • Sigmodontinae
  • Trachea
  • Transcription, Genetic
  • Transfection
  • Virus Replication
  • alpha 1-Antitrypsin

Research

keywords

  • Journal Article

Identity

Language

  • eng

PubMed ID

  • 2017680

Additional Document Info

start page

  • 431

end page

  • 434

volume

  • 252

number

  • 5004