Down-regulation of cystic fibrosis gene mRNA transcript levels and induction of the cystic fibrosis chloride secretory phenotype in epithelial cells by phorbol ester Academic Article uri icon


MeSH Major

  • Chlorides
  • Cystic Fibrosis
  • Down-Regulation
  • RNA, Messenger
  • Tetradecanoylphorbol Acetate
  • Transcription, Genetic


  • To evaluate the hypothesis that phorbol myristate acetate (PMA) might modulate the expression of the cystic fibrosis (CF) gene in epithelial cells, we examined the effect of PMA on CF mRNA levels and regulation of Cl- secretion. Strikingly, PMA down-regulated CF mRNA transcript numbers in a dose- and time-dependent manner. Importantly, in parallel with the reduction of CF mRNA levels, PMA-treated cells were unable to up-regulate Cl- secretion in a normal fashion in response to forskolin, an effect which was also dose- and time-dependent. Thus, PMA is capable of modulating expression of the CF gene and induces T84 cells to adopt the "CF phenotype" in regard to regulation of Cl- ion transport.

publication date

  • December 1991



  • Academic Article



  • eng

PubMed ID

  • 2037584

Additional Document Info

start page

  • 10319

end page

  • 23


  • 266


  • 16